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Smita Ramprakash - Manyzone Expert
EDUCATION Subjects studied: Bacteriology, Virology, Immunology, Mycology, Parasitology, Anatomy, Physiology and Biochemistry, Microbiology, Zoology, Chemistry, English and Sanskrit
Degrees
M.Sc in Medical Microbiology
B.Sc in Microbiology, Chemistry, Zoology
Experience
PREVIOUS EMPLOYMENT:


• Lecturer: Department of Biotechnology, Manipal life sciences center, Manipal – Feb 2009 to Feb 2012.

o Responsibilities: Teaching Immunology, Molecular biology and Microbiology for B.sc and M.sc biotechnology students as well as Immunology for Biotechnology Finishing School students (B.E Biotechnology). Conducting Molecular Microbiology research.

• Lecturer: (Teaching faculty on ad hock basis) Department of Microbiology and Biology KMC- International Center, Manipal, - July 2007 to December 2008.

o Responsibilities: Teaching both theoretical and practical aspects of Microbiology, Immunology as well as Biology and Genetics to Medical students..

• Research Associate Specialist: Department of Dermatology, University of Wisconsin School of Medicine, Madison, WI, USA,– Jan 2005 to March 2006.

o Responsibilities: Culturing the Human/Mouse Primary Melanocyte Culture. Managing the laboratory requirements, maintaining the purchase orders.

• Research Laboratory Assistant: Wake Forest University School of Medicine, Department of Dermatology Research, Winston- Salem, NC, USA – June2004-Dec2004.

o I received training in the field of cell and molecular biology.

• Lecturer: Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal – March 1997 to September 2001.

o Responsibilities – Teaching medical microbiology to MBBS students, preparing the schedule, preparing the question papers, conducting and evaluation of class examinations.

• Clinical Diagnostics In-charge: Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal – March 1997 to September 2001.

o Responsibilities - Diagnosis of microorganisms, conducting antibiotic sensitivity tests.

• Trainee: International Center for Health Sciences, Manipal, India- 1996 (6 months)

o Underwent training in teaching programs.

• Laboratory Teaching Faculty: Chyavana Institute of Medical Laboratory Technology, Udupi, India – 1995 September-1996 April.

o Responsibilities: Teaching medical microbiology to students of Bachelor of laboratory technology (BLT) and Master of laboratory technology (MLT) courses.

COURSES TAUGHT

Bachelor of Medicine, Medical Laboratory technology, Genetics and Biology (Pre B.Sc.), Tissue culture and maintenance of cell lines (Bachelor of Science)

SUBJECT / CHAPTER TAUGHT

General Principles of Medical Microbiology, Bacteriology, Immunology, Mycology, Virology, Parasitology, Molecular Biology, Basics of Cell biology, Genetics.

RESEARCH WORK:

Title of the research project:
1. Diagnosis of Enteric fever by multiplex PCR using Salmonella species specific primers (Standardization by culture PCR).
2. Species-Specific PCR for the identification of Mycoplasma contamination in cell line culture.

Projects Supervised:
1. Super antigens and their effects
2. Immune privilege: Pros and cons
3. Construction of homemade T/A cloning vector


TECHNIQUES USED IN MY CAREER

Microbiology - ELISA, WIDAL, VDRL, RPR, CRP, Various Bacterial Cultures,
Blood Cultures, Slide Cultures, Fungal cultures, various bacterial and fungal staining
Techniques and observation and identification of various parasites from different infectious
specimens.

Cell/Molecular Biology - Tissue culture, Western Blot, PCR, RT-PCR, Human/Mouse
primary Melanocyte Culture and cultivation of Mycoplasma and its identification by PCR and RFLP.



TRAINING UNDERGONE

• Bacteriology – Kasturba Medical College , Manipal , India – 1996
• Corneal infections – Himalayan Eye Hospital , Pokhara , Nepal - 1998
• Basic Laboratory safety - University of Wisconsin , Madison , WI , USA – 2006
• Basic Radiation Safety - University of Wisconsin , Madison , WI , USA -2006
• Mouse handling and basic surgery – University of Wisconsin , Madison , WI , USA - 2006

Description
PREVIOUS EMPLOYMENT:


• Lecturer: Department of Biotechnology, Manipal life sciences center, Manipal – Feb 2009 to Feb 2012.

o Responsibilities: Teaching Immunology, Molecular biology and Microbiology for B.sc and M.sc biotechnology students as well as Immunology for Biotechnology Finishing School students (B.E Biotechnology). Conducting Molecular Microbiology research.

• Lecturer: (Teaching faculty on ad hock basis) Department of Microbiology and Biology KMC- International Center, Manipal, - July 2007 to December 2008.

o Responsibilities: Teaching both theoretical and practical aspects of Microbiology, Immunology as well as Biology and Genetics to Medical students..

• Research Associate Specialist: Department of Dermatology, University of Wisconsin School of Medicine, Madison, WI, USA,– Jan 2005 to March 2006.

o Responsibilities: Culturing the Human/Mouse Primary Melanocyte Culture. Managing the laboratory requirements, maintaining the purchase orders.

• Research Laboratory Assistant: Wake Forest University School of Medicine, Department of Dermatology Research, Winston- Salem, NC, USA – June2004-Dec2004.

o I received training in the field of cell and molecular biology.

• Lecturer: Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal – March 1997 to September 2001.

o Responsibilities – Teaching medical microbiology to MBBS students, preparing the schedule, preparing the question papers, conducting and evaluation of class examinations.

• Clinical Diagnostics In-charge: Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal – March 1997 to September 2001.

o Responsibilities - Diagnosis of microorganisms, conducting antibiotic sensitivity tests.

• Trainee: International Center for Health Sciences, Manipal, India- 1996 (6 months)

o Underwent training in teaching programs.

• Laboratory Teaching Faculty: Chyavana Institute of Medical Laboratory Technology, Udupi, India – 1995 September-1996 April.

o Responsibilities: Teaching medical microbiology to students of Bachelor of laboratory technology (BLT) and Master of laboratory technology (MLT) courses.

COURSES TAUGHT

Bachelor of Medicine, Medical Laboratory technology, Genetics and Biology (Pre B.Sc.), Tissue culture and maintenance of cell lines (Bachelor of Science)

SUBJECT / CHAPTER TAUGHT

General Principles of Medical Microbiology, Bacteriology, Immunology, Mycology, Virology, Parasitology, Molecular Biology, Basics of Cell biology, Genetics.

RESEARCH WORK:

Title of the research project:
1. Diagnosis of Enteric fever by multiplex PCR using Salmonella species specific primers (Standardization by culture PCR).
2. Species-Specific PCR for the identification of Mycoplasma contamination in cell line culture.

Projects Supervised:
1. Super antigens and their effects
2. Immune privilege: Pros and cons
3. Construction of homemade T/A cloning vector


TECHNIQUES USED IN MY CAREER

Microbiology - ELISA, WIDAL, VDRL, RPR, CRP, Various Bacterial Cultures,
Blood Cultures, Slide Cultures, Fungal cultures, various bacterial and fungal staining
Techniques and observation and identification of various parasites from different infectious
specimens.

Cell/Molecular Biology - Tissue culture, Western Blot, PCR, RT-PCR, Human/Mouse
primary Melanocyte Culture and cultivation of Mycoplasma and its identification by PCR and RFLP.



TRAINING UNDERGONE

• Bacteriology – Kasturba Medical College , Manipal , India – 1996
• Corneal infections – Himalayan Eye Hospital , Pokhara , Nepal - 1998
• Basic Laboratory safety - University of Wisconsin , Madison , WI , USA – 2006
• Basic Radiation Safety - University of Wisconsin , Madison , WI , USA -2006
• Mouse handling and basic surgery – University of Wisconsin , Madison , WI , USA - 2006



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